| Sample ID |
Culture (24-48 hr) |
RV-PCR (9 hr) |
| Average CFU/Sample |
Source of Culture Result* |
PCR of Culture (Ct)** |
Culture Result (Pos/Neg) |
Average ΔCt |
Std Dev ΔCt |
RV-PCR Results (Pos/Neg) |
Notes |
| 1242 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1244 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1245 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1248 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1250 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1251 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1252 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1253 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1256 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1263 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1267 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1268 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1269 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1270 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1272 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1274 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1469 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
1.8E1 |
Plates, FF |
Undetermined |
Pos |
0.0 |
0.0 |
Neg |
Cross-contamination observed for culture only |
| PC |
3.6E1 |
Plates, FF |
29.7 |
Pos |
12.3 |
0.1 |
Pos |
|
| 1281 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1282 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1284 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1286 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1330 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1336 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1402 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1403 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1404 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1406 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1407 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1409 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1410 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1411 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1412 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1415 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1470 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
1.2E2 |
Plates, FF |
29.5 |
Pos |
12.1 |
0.1 |
Pos |
See Footnote (1) |
| 1249 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1418 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1422 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1423 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1426 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1427 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1428 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1429 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1434 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1435 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1436 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1437 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1440 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1442 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1449 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1471 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
3.6E1 |
Plates, FF |
15.5 |
Pos |
10.2 |
0.3 |
Pos |
See Footnote (1) |
| 1450 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1390 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1391 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1392 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1393 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1398 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1399 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1400 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1401 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1405 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1439 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1441 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1443 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1444 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1472 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
5.3E1 |
Plates, FF |
17.8 |
Pos |
7.8 |
0.6 |
Pos |
|
| 1430 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1431 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1433 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1445 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1446 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1447 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1448 |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| 1474 (TB) |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| NC |
0 |
N/A |
N/A |
Neg |
0.0 |
0.0 |
Neg |
|
| PC |
5.3E1 |
Plates, FF |
15.6 |
Pos |
7.1 |
0.1 |
Pos |
|
*Positive (pos) or negative (neg) culture determination based upon the serial dilution (plates) or filter funnel (FF) plate.
Positive culture results obtained if > 0 Bg colonies were present on serial dilution or filter membrane plates, and/or Bg-positive PCR results were obtained (Ct < 35) from colonies and/
or the EC or RS. N/A = not applicable; No CFU for analysis and EC not turbid and/or no growth from RS.
**Cycle threshold (Ct) values obtained from real-time PCR analysis of selected colonies and/or enrichment culture (EC).
Positive RV-PCR result based upon average ΔCt≥ 6 and T9 Ct ≤ 39.
Footnote (1): The PC results are from PCs prepared in different batches (spiked at either the 101 or 102 spores per wipe level).
Abbreviations: PC, positive control; NC, negative control; TB, true blank. |
