Figure 3: PAI-1 and GSTP1 gene methylation in prostate epithelial cells and prostate cancer cell lines analyzed with MSRE-qPCR assay. (A) Semi-quantitative results for GSTP1 gene methylation in normal and prostate cancer cells obtained by electrophoresis after MSRE-qPCR. (+) Digestion with HhaI, (-) control reaction without HhaI. (B) Bar chart comparing the calculated methylation index for PAI-1 gene and GSTP1 gene.