Note: The primary embryonic brain cells from 14 days pregnant female CD1 mice were parallel cultured in the same conditions (a). Cells on day-6 culturing were transfected without (b, day 3 after transfection) or with (c, day 3 after transfection) the non-viral vector 20866 (The cluster-presences appeared in this group only), which was carrying reprogramming cassette c-Myc-Klf4-Oct4- Sox2-(MKOS)-IRES-mOrange, named pCAG2LMKOSimO (vector 20866, Addgene).

Figure 1: Parallel culturing embryonic brain cells.