Note: The cluster-look iPSCs were produced during approximately day 14 and 26. They were verified by expressions of stem cell biomarkers alkaline phosphatase stain (b), stage-specific antigens SSEA-1 and GF141 on day 18 (c and d). The vector 20866 mOrange-positive marker with iPSCs achieved ultimate gene expression on roughly day 22 (f). iPSCs present significantly different morphology with the parallel control embryonic brain cells without vector 20866 (a and e) on day 18 and 22.

Figure 2: Producing iPSCs by reprogramming genes conversion of embryonic brain cells.