| Note: The original embryonic brain cells without reprogramming genes were
differentiated into progressive staging to form Progenitor neuron cells in 83 days
(a), Neurons in 86 days (b), and Neuron network in 127 days (c), respectively.
However, there appeared significant rapidity up staging for transformation of
reprogrammed iPSCs to Progenitor neuron cells to procedure approximately
26 days (d), Mature neuron cells in 31 days (e), Reprogramming genes and
neurotrophic factors fuel stem cells and Neuron network in 86 days (f),
correspondingly. The analysis of quantitative results was carried on by using
Figure 3A-g, -h and -i to compare with Figure 3A-a and -d (Progenitor neurons),
-b and -e (Neurons), as well as –c and -f (Neural network) as their groups of nonreprogrammed
cells vs reprogrammed cells, respectively. These reprogrammed
neuron cells were further confirmed by the mature neuronal marker MAP2 (a),
and tyrosine hydroxylase (TH) as the rate-limiting enzyme in dopamine synthesis
(b) on Day 50 in the Figure 3B. |
