| Note: The early staged readily reprogrammed neuron cells on Day 27 were
co-transfected with/without complete length cDNA BDNF. Amount of early
Reprogramming genes and neurotrophic factors fuel stem cells neuronal
marker doublecortin (~40 kDa) showed robust expressions in producing neuron
cells in the groups of cDNA BDNF plus vector 20866, or vector 20866 alone,
when compared with the control group (vehicle) by the western blotting (a).
The data represent mean values of three independent experiments, suggesting
significant differences between the groups of cDNA BDNF plus vector 20866
and vector 20866 alone or control; cDNA BDNF alone and control; P value
<0.05 (b). β-Actin was as the loading control for each group. After densitometry,
integrated density value (IDV) for each protein band was determined and
normalized levels of β-Actin. |
