Figure 3: PCR was conducted with the use of formalin-fixed paraffin-embedded samples. The PCR products were ligated and cloned into T-vector, and sequenced. No somatic mutation from surrounding normal tissues was observed in either sample (A, case 1, and C, case 2). In Case 1, a deletion (c.1956delG) with missense mutation (c.1958 C>T) causing frameshifted-nonsense mutation (p.G652GfsX3) in exon 14 (B) is observed (red box). In Case 2, a missense mutation (c.2516 T>C, p.L839P) in exon 18 (D) is noted (red box). Note that sequence data in case 2 (C and D) are arranged in reverse direction.