Variable Value Inlet protein concentration c0 =7.14 × 10−3 mol/m3 Flow-rate F=1.67 × 10−8 m3/s Column length L=0.014, 0.027, 0.041, 0.104m Column diameter CD=0.01m Bed porosity ε =0.39 Bead porosity εi=0.75 Bead radius rm=5 × 10−5 m Axial dispersion DL=5.75 × 10−8 m2/s Film mass-transfer rate kf=6.9 × 10−6 m/s Solution viscosity μ=0.95 g/m.s Solution density ρ=1.0 × 106 g/m3 Lysozyme diffusivity in free liquid DAB= 1.06 × 10−10 m2/s Effective diffusion coefficient DE=5.3 × 10−11 m2/s Adsorption rate constant (Transport model) k1 =1.144m3/(mol s) Adsorption rate constant (Thomas model) k1=0.286m3/(mol s) Equilibrium desorption constant Kd =1.748 × 10−3 mol/m3 Maximum adsorption capacity qms=1.0 mol/m3 Maximum adsorption capacity of solid gel qm =5.246 mol/m3

Table 1: Base case data used in simulation studies of frontal affinity adsorption of lysozyme to Cibacron Blue Sepharose CL-6B [5,8,13].