Phloretin concentration (µg/ml)        
Added Observed   R.S.D (%)   Bias (%)  
  Within-day Between-day Within-day Between-day Within-day Between-day
0.5 0.546 0.546 9.62 10.9 9.22 9.18
1.0 1.02 0.96 3.94 9.77 2.11 -3.58
5.0 4.32 4.61 5.47 13.6 -13.5 -7.75
10 9.52 9.21 4.20 7.99 -4.79 -7.95
50 52.1 52.3 1.09 4.94 4.25 4.60
100 99.0 99.0 0.31 1.16 -1.01 -0.98
Table 1: Within- and between-day precision and accuracy of the assay for phloretin in rat serum (n = 5, mean, R.S.D., and Bias).