No Chromatographic condition Parameters of absorption peaks Estimate of the results 1 30%B+70%A changed to 45%B+55%A in 10 min retention time: 1.946 resolution: 1.210 symmetry factor: 0.951 theoretical plate: 3408 The retention time is short, and a certain degree of separation is obtained. 2 27%B+73%A changed to 45%B+55%A in 10 min retention time: 3.051 resolution: 1.365 symmetry factor: 0.996 theoretical plate: 4068 The retention time is short，and a certain degree of separation is obtained. 3 20%B+80%A changed to 50%B+50%A in 10 min retention time: 6.403 resolution: 1.610 symmetry factor: 0.993 theoretical plate: 4745 The retention time is proper, and good resolution is obtained. 4 30%B+70%A , isocratic elution, 0-15 min The sample is remained in the column. The sample is retained in  the column 5 35%B+65%A, isocratic elution, 0-15 min, retention time: 2.152 resolution: 1.214 symmetry factor: 0.966 theoretical plate: 2055 The retention time is too short, and the resolution is not good.

Table 1: The chromatographic conditions and results. A is 0.05% TFA aqueous solution and B is acetonitrile. The sample which was injected into the HPLC system in this study is CMS (0.5 mg/mL). The parameters are the values from measurements of CMS E1.