| HPLC |
|
TLC-UV Densitometry |
| Parameter |
A |
G |
Reference values |
Parameter |
A |
G |
| retention time tR |
16.3 ± 0.1 |
10.3 ± 0.1 |
|
Rf |
0.14 |
0.66 |
| Resolution Rs |
9.86 |
2.63 |
Rs≥2 |
|
Resolution Rs |
|
7.7 (calculated between A and G)
2.27
(calculated between G and AD) |
| Tailing Factor |
1.11 |
1.2 |
T≤2 |
|
Capacity Factor (K') |
3.5 |
22.75 |
| Asymmetry Factor |
1.33 |
0.98 |
|
|
Selectivity α |
|
6.50 |
| Capacity Factor (K') |
6.13 |
3.52 |
1<K'<10 |
|
Tailing Factor |
1.25 |
1 |
| Selectivity α |
1.33 |
1.74 |
α>1 |
|
|
|
|
| Column Efficiency N |
2988.4 |
4261 |
N>2000 |
|
|
|
|
| HETP (Height Equivalent to theoretical plate) |
0.084 |
0.059 |
|
|
|
|
|
|
