Proposed methods for Ambroxol HCl Reported methods for A Proposed methods for Guaiphenesin Reported methods for G HPLC TLC PLS PCR Reporteda HPLC TLC PLS PCR Reportedb Mean 99.95 99.89 100.42 100.39 100.22 100.07 100.00 100.25 100.29 100.28 RSD 0.821 1.029 1.093 1.094 0.754 1.093 1.161 1.419 1.531 1.066 Conc. Range 5-80 µg.-1 1-7 µg.-1 6-9 µg.-1 6-9 µg.-1   10-150 µg/band 2-10 µg/band 40-60 µg.-1 40-60 µg.-1   n 5 7 8 8 4 6 9 8 8 7 Variance 0.674 1.059 1.195 1.197 0.569 1.195 1.348 2.014 2.344 1.136 Student's t test 0.508 (2.365)* 0.556 (2.262)* 0.325 (2.230)* 0.325  (2.23)*   0.350 (2.201)* 0.496 (2.145)* 0.046 (2.600)* 0.014 (2.600)*   F-test 1.185 (9.117)* 1.861 (8.941)* 2.100 (8.887)* 2.104 (8.887)*   1.052 (4.387)* 1.187 (4.147)* 1.773 (4.207)* 2.063 (4.207)*

n is the number of trials. * These values represent the corresponding tabulated values of t and F at p=0.05. a.Reported method was a spectrophotometric method for A by measuring the absorbance of A in 0.1M HCl at 244 nm. b.HPLC for the determination of G on C18 column, mobile phase (KH2PO4-methanol, 55:45; v/v, pH3), Flow rate 1 ml/min UV detection at 220 nm.

Table 6: Statistical comparison of the results obtained by the proposed methods and the reference reported methods for pure samples of Ambroxol HCl and Guaifenesin.