Biological Matrix
Added (μg/ml)
Vorinostat concentration (μg/ml)
Observed (mean)
RSD(%)
Bias (%)
Within-day
Between-day
Within-day
Between-day
Within-day
Between-day
Serum
0.05
0.051
0.046
14.28
12.91
–3.31
–8.31
0.5
0.43
0.46
6.17
14.81
14.40
–8.66
1
0.93
0.99
1.86
6.22
–7.30
–0.84
5
5.37
5.13
2.73
3.50
7.3
2.63
10
9.71
9.94
1.55
2.69
–2.90
–0.65
50
50.22
50.05
0.290
0.13
0.44
0.09
Urine
0.5
0.46
0.43
10.88
5.41
–8.28
–14.35
1
0.96
0.91
5.32
9.70
–4.49
–9.13
5
5.64
5.08
13.65
6.08
12.72
1.63
10
10.47
9.98
2.14
4.72
4.72
–0.18
12.5
12.94
12.69
1.01
3.39
3.56
1.52
25
25.83
24.88
0.84
0.78
3.32
–0.47
Table 1:
Within- and between-day precision and accuracy of the assay for vorinostat in rat serum and urine (n = 6, mean, RSD and bias).
