Biological Matrix Vorinostat concentration (μg/ml) %Recovery (Extraction efficiency) %Recovery (Freeze-Thaw stability) %Recovery (Auto-injector stability) Serum 0.05 99.83 ± 2.74 99.10 ± 4.55 98.74 ± 3.58 5 100.13 ± 1.37 97.56 ± 2.08 99.83 ± 7.29 50 101.37 ± 3.32 99.70 ± 3.89 100.50 ± 7.47 Urine 0.5 99.92 ± 3.61 97.48 ± 3.17 99.43 ± 5.19 5 97.45 ± 0.10 100.21 ± 1.80 99.87 ± 4.43 25 100.22 ± 3.28 98.02 ± 1.23 99.05 ± 4.89

Table 2: Recovery and stability of vorinostat in rat serum and urine (n = 3, mean, and SD).