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Figure 2: A) HPLC chromatogram of Aspergillus Sojaeelicited soybean extracted (Crude Sample). Column: reverse C18 column (4.6mm 250mm, 5�m); themobilephase A: acetic, B: acetonitrile Elution was carried out at a flow rate of 1.0mL/min with the following solvent system: A=acetic acid/water (pH3.0); B=acetonitrile; 0% B to 45% B in 17min, then 45% B to 90% B in 10min followed by holding at 90% B for 6min. Detection: 285nm; flow rate: 1.0mL/min. B) HPLC Chromatogram of crude ethanol extract of un-elicited soy bean seed which served as control. |