Figure 3: HSCCC chromatogram of the crude sample and HSCCC separated peak fractions from elicited Soybean seeds, Peak E at a retention time (RT) of 260–310 min from the HSCCC chromatogram gave a corresponding peak of glyceollins. A two phase solvent system composed of n-hexane ethyl acetate-methanol-water- (3:4:2:1v/v) was applied for the HSCCC separation with the upper phase as the stationary phase and the lower phase as the mobile phase. Detection by UV:285nm. Total volume: 325mL; sample loop: 25mL; revolution speed: 800rpm. Stationary phase: upper organic phase; elution mode in the coil system: ’headtotail’; flow rate: 1.0mL min).