Figure 6: A) Positive ion electro spray ionization-MS analysis of glyceollins isolated from soybean cotyledons inoculated with A. sojae. Separated by HSCCC. (ESI) Voltage of 3.50 KV, Ion source temperature is 120� C and desolvation temperature is 250� C. B) UPLC�MS analysis of glyceollins isolated from soybean cotyledons inoculated with A. sojae , Separated by HSCCC and analyzed by HPLC. UPLC separation was achieved using a Waters Acquity HSS T3 Column (100 � 2.1 mm i.d., 1.8 �m particle size).