Figure 6: A) Positive ion electro spray ionization-MS analysis of glyceollins isolated from soybean cotyledons inoculated with A. sojae. Separated by HSCCC. (ESI) Voltage of 3.50 KV, Ion source temperature is 120° C and desolvation temperature is 250° C. B) UPLC–MS analysis of glyceollins isolated from soybean cotyledons inoculated with A. sojae , Separated by HSCCC and analyzed by HPLC. UPLC separation was achieved using a Waters Acquity HSS T3 Column (100 × 2.1 mm i.d., 1.8 µm particle size).