Table 1

Column			Pore diameter (nm)	Pore volume (mL/g)	Surface area (m²/g)	Carbon load (%)	HPLC separation
Symbol	Silica (type)	Trade name	Pore diameter (nm)	Pore volume (mL/g)	Surface area (m²/g)	Carbon load (%)	HPLC separation
A	C1	Wakosil 5TMS	12	1.0	300	4	Not Separated (NS)^c
B	C4	Mightysil RP-4 GP	12.5	1.1	350	4	NS
C	C18	Mightysil RP-18 GP Aqua	13.5	0.9	270	15	NS
D	C18	Inertsil ODS-4	10	1.05	450	11	NS
E	Alkyl diol	Inertsil HILIC (alkyl diol)	10	1.05	450	20	Completely separated

^a150×4.6 mm; d_p= 5 μm: data supplied from manufactures.
^bMobile phase of water; column temperatures ≥ 25°C; flow-rates ≥ 0.5 mL/min; HPLC retention times ≤ 15 min.
^c Not separated: between MEL and the interfering milk extract.

Table 1: Physical/chemical specifications of the reversed-phase columnsa used and chromatographic CYR and MEL separations obtained under the HPLC conditions examined^b .