Table 2

Parameter	CYR	MEL
Standard stability^a: At 25°C for 24 h At 5°C for 10 days	101.0 (0.6) 98.6 (0.8)	99.3 (0.9) 100.6 (1.0)
Linearity (r^b)	0.991	0.994
Accuracy^c	93.2 – 97.8	94.5 – 99.1
Precisiond	≤ 2.8	≤ 2.4
Sensitivity (QL^e)	8.5	10

^a The chromatographic peak area (%) after completion of the store time: data are expressed as averages (n=5, for each compound); relative standard deviation (RSD) in parentheses (%).
^b r is the correlation coefficient (P< 0.01): mean of three determinations using spiked milk samples for calibration curves: ranges of concentrations were 20–1,000 ng/mL for CYR and 20 – 3,000 ng/mL for MEL, respectively.
^c Average recoveries (%) from six replicates at three QC levels (QC1 = 30 ng/mL for CYR and 50 ng for MEL; QC2= 100 ng/mL for both analytes; QC3 = 500 ng/ mL for CYR and 250 ng/mL for MEL).
^d Values are RSDs (%, n=6 of each level).
^e Quantitation limit (ng/mL), QL as the concentration of analyte giving a signalto- noise ratio > 10.

Table 2: The main method validation data.