Figure 1: Purification of Ostrich IgY by affinity chromatography. The figure shows in (A) an SDS-PAGE of the ostrich IgY purified by the Polson method, showing the light and the heavy chains of 30 and 70 kDs respectively, and the livetins fraction, which are the proteins in between the light and heavy chain of the ostrich IgY. Western blotting of purified ostrich IgY by affinity chromatography (B) probed with a SpLA-HRP conjugate confirms electrophoresis results in the number of protein bands of light (30 kDa) and heavy chains (70 kDa).