Sample type

Concentration added ngml-1

Intraday (n=6)

Interday (n=6)

Concentration found (ngml-1) (mean ±sd)

Precision
(% RSD)

Accuracy (%RE)

Concentration found (ngml-1) (mean ±sd)

Precision
(% RSD)

Accuracy (%RE)

SLN

250

240 ± 10

4.2

4.0

244 ± 10

4.1

2.4

500

490 ± 12

2.4

2.0

502 ± 12

2.4

-0.4

1000

998 ± 32

3.2

0.2

986 ±  3

0.3

1.4

5000

4980 ± 12

0.2

0.4

4985 ± 19

0.4

0.3

10000

9960 ± 23

0.2

0.4

9992 ± 12

0.1

0.1

Plasma

250

239 ± 11

4.6

4.4

247 ± 10

4.0

1.2

500

498 ± 23

4.6

0.4

503 ± 14

2.8

-0.6

1000

990 ± 42

4.2

1.0

999 ± 17

1.7

0.1

5000

4986 ± 12

0.2

0.3

4989 ± 13

0.3

0.2

10000

9966 ± 50

0.5

0.3

9994 ± 12

0.1

0.1

Brain

250

247 ± 12

4.9

1.2

252 ± 2

0.8

-0.8

500

505 ± 12

2.4

-1.0

498 ± 13

2.6

0.4

1000

999 ± 29

2.9

0.1

991 ± 21

2.1

0.9

5000

4988 ± 63

1.3

0.2

4980 ± 21

0.4

0.4

10000

9999 ± 22

0.2

0.0

9978 ± 18

0.2

0.2

Liver

250

254 ± 7

2.8

-1.6

252 ± 11

4.4

-0.8

500

510 ± 13

2.5

-2.0

490 ± 10

2.0

2.0

1000

1010 ± 12

1.2

-1.0

1002 ± 21

2.1

-0.2

5000

4992 ±  34

0.7

0.2

4997 ± 39

0.8

0.1

10000

9993 ± 46

0.5

0.1

9990 ± 21

0.2

0.1

Kidney

250

251 ± 4

1.6

-0.4

249 ± 8

3.2

0.4

500

489 ± 8

1.6

2.2

510 ± 12

2.4

-2.0

1000

1004 ± 23

2.3

-0.4

998 ± 34

3.4

0.2

5000

5012 ± 34

0.7

-0.2

4988 ± 23

0.5

0.2

10000

9989 ± 54

0.5

0.1

10010 ± 34

0.3

-0.1

Table 1: Precision and accuracy of the method.