UPLC
Actual concentration (μgmL-1)
Intra-day
Inter-day
Measured concentration
± S.D.
R.S.D. (%)
10
9.9610
0.0894
0.8975
9.9415
0.0515
0.5180
20
20.0967
0.1701
0.8464
20.1250
0.1450
0.7205
30
29.7920
0.1514
0.5083
29.9530
0.1370
0.4574
40
39.9057
0.2069
0.5186
39.8585
0.1515
0.3801
50
49.8837
0.1872
0.3752
49.8855
0.2045
0.4099
HPLC
30.101
0.096
0.319
30.138
0.046
0.153
60
59.940
0.217
0.362
60.011
0.273
0.455
90
89.907
0.303
89.955
0.308
0.342
120
120.143
0.169
0.141
120.350
0.263
0.218
150
150.448
0.508
0.338
150.596
0.354
0.235
