UPLC ( n=4) |
Concentration
(μgmL-1) |
acetonitrile : H2O (78:22 v/v) |
acetonitrile : H2O (82:18 v/v) |
Measured
concentration |
± S.D., |
R.S.D (%) |
Measured
Concentration |
± S.D., |
R.S.D. (%) |
10 |
9.948 |
0.042 |
0.422 |
9.934 |
0.051 |
0.514 |
20 |
20.120 |
0.150 |
0.746 |
20.143 |
0.121 |
0.598 |
30 |
30.019 |
0.182 |
0.608 |
30.025 |
0.188 |
0.626 |
40 |
40.132 |
0.412 |
1.027 |
40.109 |
0.407 |
1.014 |
50 |
50.020 |
0.298 |
0.596 |
49.954 |
0.237 |
0.474 |
HPLC( n=4) |
Concentration
(μgmL-1) |
Acetonitrile : 10mM Amm. acetate
(83:17 v/v) |
Acetonitrile : 10 mM Amm. acetate
(87:13 v/v) |
Measured
concentration |
± S.D., |
R.S.D.(%) |
Measured
concentration |
± S.D., |
R.S.D. (%) |
30 |
30.225 |
0.299 |
0.989 |
30.262 |
0.303 |
1.002 |
60 |
59.940 |
0.217 |
0.362 |
60.077 |
0.160 |
0.266 |
90 |
89.996 |
0.127 |
0.141 |
89.877 |
0.442 |
0.492 |
120 |
120.236 |
0.328 |
0.272 |
120.810 |
0.767 |
0.635 |
150 |
150.226 |
0.196 |
0.130 |
150.573 |
0.315 |
0.209 |
UPLC = Ultra performace liquid chromatography
HPLC = High performance liquid chromatography
n = number of replicates |
