| S.No |
Column Used |
Mobile Phase |
Parameter
optimised |
Inj. Vol. |
Observation |
Flow rate |
Results |
| 2a |
Kromasil Eternity
C-18 (50×2.1 mm)
2.5 μm |
Ammonium acetate
(PH 6.7): CAN
(gradient) |
Flow rate and
gradient |
5 μl |
Pear shape not good,
More run time |
0.25ml/min |
Method rejected |
| 2b |
Kromasil Eternity
C-18 (50×2.1 mm)
2.5 μm |
Ammonium acetate
(PH 6.7): CAN
(gradient) |
gradient |
5 μl |
Tailing observed |
0.35ml/min |
Method rejected |
| 2c |
Kromasil Eternity
C-18 (50×2.1 mm)
2.5 μm |
Ammonium acetate
(PH 6.7): CAN
(gradient) |
gradient |
3 μl |
Longer run time |
0.35ml/min |
Method rejected |
| 2d |
Kromasil Eternity
C-18 (50×2.1 mm)
2.5 μm |
Ammonium acetate
(PH 6.7): CAN
(gradient) |
gradient |
2 μl |
Peak shape good |
0.35ml/min |
Method accepted |
