Analytes

Precision

Repeatability

Stability

Intra-day (n=6)

Inter-day (n=6)

Average peak area

R.S.D. (%)

Average peak area

R.S.D. (%)

Mean (mg/g)

R.S.D. (%)

Average peak area

R.S.D. (%)

Chlorogenic Acid

50.80

0.35

52.30

2.33

0.27

2.97

51.40

1.58

Gallic Acid

561.95

0.20

546.22

2.36

1.96

1.37

561.00

0.24

Paeoniflorin

788.30

0.06

791.62

0.66

7.75

1.03

793.50

1.07

Jatrorrhizine Hydrochloride

202.50

0.59

205.60

0.66

0.49

2.49

202.90

1.68

Dehydroevodiamine

77.02

1.96

78.46

0.70

0.32

2.76

77.80

2.02

Palmatine Chloride

625.90

0.29

628.63

0.75

2.07

2.84

626.20

0.95

Berberine Hydrochloride

2295.75

0.12

2310.46

0.77

6.78

2.25

2304.40

0.73

Table 1: Precision, repeatability and stability of the HPLC method for determination of the seven analytes.