Analytes

Sample

Concentration

Mean Recovery (%) ± R.S.D

Sample Content (mg)

Added (mg)

Found (mg)

Recovery (%)

 

S1a

 

0.3392

0.7733

103.39

 

Gallic Acid

S2b

0.4226

0.4240

0.8651

104.35

102.99 ± 1.45

 

S3b

 

0.5088

0.9376

101.22

 

 

S1

 

0.0689

0.1567

102.35

 

Chlorogenic Acid

S2

0.0862

0.0862

0.1770

101.99

102.39 ± 1.85

 

S3

 

0.1034

0.1903

102.82

 

 

S1

 

1.2040

2.7621

104.40

 

Paeoniflorin

S2

1.5050

1.5050

3.0820

104.78

103.84 ± 1.35

 

S3

 

1.8061

3.3536

102.35

 

 

S1

 

0.0857

0.1965

96.18

 

Jatrorrhizine Hydrochloride

S2

0.1071

0.1071

0.2212

95.67

96.19 ± 1.13

 

S3

 

0.1285

0.2337

96.72

 

 

S1

 

0.0566

0.1303

100.28

 

Dehydroevodiamine

S2

0.0707

0.0707

0.1403

98.42

99.19 ± 2.99

 

S3

 

0.0848

0.1546

98.87

 

 

S1

 

0.3520

0.8004

102.40

 

Palmatine Chloride

S2

0.4400

0.4400

0.8648

96.56

100.11 ± 2.79

 

S3

 

0.5279

0.9752

101.39

 

 

S1

 

1.0890

2.4853

104.64

 

Berberine Hydrochloride

S2

1.3691

1.3613

2.7122

98.67

102.63 ± 2.93

 

S3

 

1.6335

3.0775

104.59

 
Recovery (%) = (found−original) ×100 /added. The results indicated that the developed method was reliable and accurate for the measurement of the seven analytes.
aThe samples added known amounts of standards at low level (80% of the known amounts).
bThe samples added known amounts of standards at medium level (same as the known amounts).
cThe samples added known amounts of standards at high level (120% of the known amounts).
Table 2: Recovery test of the seven analytes.