Table 1

Purification step	Group	Total protein (mg)	Total activity (mmol/min)	Specific activity (mmol/min/ml)	Yield (%)	Purification fold
1- Crude extract	Group (1)	572.29	174.54	0.305	100	1
2- GSH affinity	Group (1)	8.61	111.88	12.995	64.11	42.61
1- Crude extract	Group (2)	461.2	106.53	0.231	100	1
2- GSH affinity	Group (2)	6.71	67.87	10.112	63.67	43.76
1- Crude extract	Group (3)	585.91	311.11	0.531	100	1
2- GSH affinity	Group (3)	10.71	167.55	15.645	53.83	29.46
1- Crude extract	Group (4)	577.14	289.14	0.501	100	1
2- GSH affinity	Group (4)	10.11	146.83	14.524	50.78	28.99
1- Crude extract	Group (5)	592.36	342.97	0.579	100	1
2- GSH affinity	Group (5)	11.24	182.32	16.221	53.15	28.01
1- Crude extract	Group (6)	589.66	331.38	0.562	100	1
2- GSH affinity	Group (6)	11.44	176.75	15.451	53.33	27.49

i- Specific activity = Total activity / Total protein.
ii- Total activity = Specific activity x Total protein.
iii- Purification fold = Specific activity of purified enzyme / Specific activity of crude enzyme.
iv- Yield (%) = (Total activity of purified enzyme / Total activity of crude enzyme) x 100.

Table 1: Purification profile of Glutathione-S-transferase enzyme in different groups.