Spiked concentration  (μg/mL)

 ACN: Phosphate buffer(58:42)

ACN: Phosphate buffer (62:38)

Measured concentration

± S.D.,

R.S.D. (%)

Measured concentration

± S.D.,

R.S.D. (%)

20

20.160

0.078

0.387

20.117

0.155

0.771

40

40.086

0.161

0.402

40.119

0.392

0.977

60

60.034

0.311

0.518

59.917

0.276

0.462

ACN=Acetonitrile
n=Number of determination.

Table 4: Data for robustness (n=3).