Parameter

No. of theoretical plates (N)

Resolution (Rs)

Relative retention (α)

ASC

SAL

pH of mobile phase

2.5

973

5069

6.80

1.71

3.0

1362

6128

10.34

2.12

3.5

1011

2796

7.62

2.08

4.0

1708

7066

6.00

1.69

4.5

961

4679

5.80

1.65

5.0

576

1220

4.07

1.75

Ratio of A: B*

40: 60

1643

4822

14.10

2.86

50: 50

1457

3355

9.18

2.17

55: 45

1665

5069

5.88

1.71

60: 40

858

2776

4.48

1.57

Conc. of H3PO4 (M)

0.01

961

1744

4.71

1.68

0.02

1030

4032

5.34

1.71

0.03

1223

4391

5.88

1.62

0.04

1365

2663

6.00

1.69

Flow rate (mL/ min)

0.8

1886

5094

7.80

1.72

1.0

1708

7066

6.00

1.69

1.2

1346

5890

5.44

1.56

*A: methanol.
B: 0.03 M phosphate buffer.
Table 1: Optimization of the chromatographic conditions for separation of ASC and SAL by the proposed method.