Parameter
No. of theoretical plates (N)
Resolution (Rs)
Relative retention (α)
ASC
SAL
pH of mobile phase
2.5
973
5069
6.80
1.71
3.0
1362
6128
10.34
2.12
3.5
1011
2796
7.62
2.08
4.0
1708
7066
6.00
1.69
4.5
961
4679
5.80
1.65
5.0
576
1220
4.07
1.75
Ratio of A: B*
40: 60
1643
4822
14.10
2.86
50: 50
1457
3355
9.18
2.17
55: 45
1665
5.88
60: 40
858
2776
4.48
1.57
Conc. of H3PO4 (M)
0.01
1744
4.71
1.68
0.02
1030
4032
5.34
0.03
1223
4391
1.62
0.04
1365
2663
Flow rate (mL/ min)
0.8
1886
5094
7.80
1.72
1.0
1.2
1346
5890
5.44
1.56