Table 1

Parameter		No. of theoretical plates (N)		Resolution (R_s)	Relative retention (α)
Parameter		ASC	SAL	Resolution (R_s)	Relative retention (α)
pH of mobile phase	2.5	973	5069	6.80	1.71
	3.0	1362	6128	10.34	2.12
	3.5	1011	2796	7.62	2.08
	4.0	1708	7066	6.00	1.69
	4.5	961	4679	5.80	1.65
	5.0	576	1220	4.07	1.75
Ratio of A: B*	40: 60	1643	4822	14.10	2.86
	50: 50	1457	3355	9.18	2.17
	55: 45	1665	5069	5.88	1.71
	60: 40	858	2776	4.48	1.57
Conc. of H₃PO₄ (M)	0.01	961	1744	4.71	1.68
	0.02	1030	4032	5.34	1.71
	0.03	1223	4391	5.88	1.62
	0.04	1365	2663	6.00	1.69
Flow rate (mL/ min)	0.8	1886	5094	7.80	1.72
	1.0	1708	7066	6.00	1.69
	1.2	1346	5890	5.44	1.56

^*A: methanol.
B: 0.03 M phosphate buffer.

Table 1: Optimization of the chromatographic conditions for separation of ASC and SAL by the proposed method.