Parameters |
SD of peak area |
%RSD |
Mobile phase composition |
|
|
Actone-Water-Acetic acid 4:2:0.2 (v/v) |
97.31 |
0.83 |
Actone-Water-Acetic acid 3.5:2.5:0.2 (v/v) |
83.99 |
0.73 |
Mobile phase volume |
|
|
6.02 mL |
125.17 |
1.07 |
10.02 mL |
80.51 |
0.70 |
Development distance |
|
|
7 cm |
121.23 |
1.06 |
7.5 cm |
123.47 |
1.08 |
8 cm |
108.25 |
0.94 |
Relative humidity |
|
|
55 |
146.17 |
1.25 |
65 |
221.43 |
1.93 |
Duration of saturation |
|
|
25 min |
206.90 |
1.79 |
30 min |
48.94 |
0.43 |
35 min |
105.57 |
0.93 |
Activation of prewashed TLC plates |
8 min |
60.21 |
0.52 |
10 min |
68.23 |
0.59 |
12 min |
111.32 |
0.95 |
Time from spotting to chromatography |
89.52 |
0.77 |
Time from chromatography to scanning |
73.98 |
0.64 |
