Parameter

Data

Linearity range (ng  spot-1)

200 - 1200

Correlation coefficient

0.9992

Limit of detection (ng  spot-1)

17.12

Limit of quantitation (ng spot-1)

51.88

Recovery (n = 9)

100.29

Ruggedness (% R.S.D.)

Analyst I

0.011

Analyst II

0.013

Precision (%R.S.D.)

Repeatability of application (n = 6)

0.10

Inter-day (n = 6)

0.73

Intra-day (n = 6)

0.37

Robustness

Robust

Specificity

specific

Table 5: Summary of validation parameter.