Method parameter/Condition

Deliberate changes

%RSD of peak area (n = 3)

PCM

DOM

TMD

Plate activation time(a)

20 min

1.23

1.52

1.48

30 min

1.33

1.49

1.68

Chamber saturation time(a)

16 min

1.59

1.27

1.74

24 min

1.29

1.33

1.39

Volume of mobile phase(b)

8.2 Ml

1.37

1.46

1.25

10.0 mL

1.91

1.93

1.73

Development distance from spot application(c)

7.7 cm

1.81

1.86

1.61

6.3 cm

1.87

1.47

1.35

(a) ±20% change in set time.
(b) ±10% change in set volume.
(c) ±10% change in set distance.
Table 4: Results from the robustness study of method.