Parameter |
NEB |
HCTZ |
S.D. of Peak Area |
% R.S.D. |
S.D. of Peak Area |
% R.S.D |
Mobile Phase Composition |
Toluene: ethyl acetate: methanol: ammonia
(3.2:2.9:1.9:0.1) |
67.14 |
1.78 |
145.80 |
1.25 |
Toluene: ethyl acetate: methanol: ammonia
(2.8:2.5:1.5:0.1) |
50.08 |
1.34 |
133.44 |
1.16 |
Mobile Phase Volume |
7.5 ml |
74.30 |
1.95 |
140.25 |
1.21 |
15 ml |
51.43 |
1.37 |
150.05 |
1.29 |
Development distance |
8 cm |
69.38 |
1.84 |
128.76 |
1.11 |
8.5 cm |
50.17 |
1.33 |
64.20 |
0.56 |
9 cm |
39.37 |
1.01 |
105.09 |
0.90 |
Duration of saturation |
|
20 min |
67.75 |
1.72 |
142.84 |
1.24 |
25 min |
54.69 |
1.38 |
65.65 |
0.56 |
30 min |
49.57 |
1.28 |
110.06 |
0.95 |
Activation of prewashed plates |
5 min |
76.97 |
1.98 |
193.34 |
1.66 |
10 min |
64.52 |
1.71 |
197.03 |
1.70 |
15 min |
61.81 |
1.69 |
161.87 |
1.39 |
Time from spotting to chromatography |
30 min |
65.71 |
1.75 |
119.17 |
1.02 |
60 min |
63.80 |
1.70 |
170.42 |
1.47 |
Time from chromatography to scanning |
30 min |
47.48 |
1.25 |
131.04 |
1.12 |
60 min |
71.68 |
1.91 |
145.70 |
1.25 |