Parameter

NEB

HCTZ

Linearity range (ng /spot )

500 - 3000

1000 - 6000

Correlation coefficient

0.9995

0.9991

Limit of detection (ng /spot )

73.58

101.12

Limit of quantitation (ng/ spot)

223.09

309.46

% Recovery (n = 9)

1.45

1.62

Ruggedness  (%R.S.D.)

Analyst I  (n = 6)

1.88

1.82

Analyst II  ( n = 6)

1.85

1.65

Precision (%R.S.D.)

Repeatability (n = 6)

0.73

1.21

Inter-day (n = 3)

1.25 - 1.77

0.39 - 0.65

Intra-day (n = 3)

0.76 - 1.53

0.34 - 0.63

Robustness

Robust

Robust

Specificity

Specific

Specific