Parameter
NEB
HCTZ
Linearity range (ng /spot )
500 - 3000
1000 - 6000
Correlation coefficient
0.9995
0.9991
Limit of detection (ng /spot )
73.58
101.12
Limit of quantitation (ng/ spot)
223.09
309.46
% Recovery (n = 9)
1.45
1.62
Ruggedness (%R.S.D.)
Analyst I (n = 6)
1.88
1.82
Analyst II ( n = 6)
1.85
1.65
Precision (%R.S.D.)
Repeatability (n = 6)
0.73
1.21
Inter-day (n = 3)
1.25 - 1.77
0.39 - 0.65
Intra-day (n = 3)
0.76 - 1.53
0.34 - 0.63
Robustness
Robust
Specificity
Specific