Figure 3b: Effect of various flow rates on the male urinary profile obtained using ZIC HILIC column (2.1 mm × 150 mm × 5 μm). Mobile phases used were- 50 mM ammonium acetate: acetonitrile (95:5 v/v) and 50 mM ammonium acetate: water: acetonitrile (50:45:5 v/v). The gradient program is described in Table 1.