Figure 7: PLS-DA plot showing variation between the samples analyzed after each freeze and thaw cycle. Five freeze and thaw cycles showed variation within 95% confidence of Hotelling T2 with one outlier seen in freeze thaw cycle 2. The X-axis represented t [1] which shows score t [1] for PC1 showing maximum variation in the dataset. The Y-axis represented t [2] which represents score t [2] for PC2 showing maximum variation not seen from just PC1. The coloured key represents each freeze and thaw cycle after which the samples were analyzed.