Figure 2: An example of a typical PCA plot based on real data where paracetamol has been spiked into a normal human urine samples. The spiked and non-spiked samples were analysed blind on the Shimadzu Trap-ToF MS, data processed using Shimadzu Profiling Solutions and exported to SIMCA P+ for multivariate analysis. Two clusters were identified where among the 12,000+ ions (pre-extraction of noise and variable ions) which matched the partition of the samples precisely to spiked and un-spiked urine: the differentiation being the presence of m/z ions and retention characteristics corresponding to paracetamol.