ak’A = tR - tM / tM (where k’A is the capacity factor, tR is the retention time of the analyte and tM is the time taken for the mobile phase to pass through the column). bRs = 2[(tR)B - (tR)A] (Rs is the resolution factor, (tR)B is the RT of peak B, (tR)A is the RT WA+WB of peak A and WA & WB is the peak width of peak A and peak B, respectively). |
||||||||||||||||||||||||
Table 2: Retention time, resolution coefficient and efficiency factor of the five dyes using present mobile phase. |