Colours Average retention time (min) * s** (min) RSD (%) Average  peak area* (x103) s ** (peak area)×103 RSD (%) Dimethyl yellow 3.946 0.065 1.64 1307 17.4 1.33 Sudan I 4.770 0.092 1.92 632 2.73 3.44 Sudan II 7.041 0.189 2.69 333 8.54 2.56 Sudan III 11.277 0.360 3.19 421 20.9 4.97 Sudan IV 14.621 0.341 2.34 311 14.2 4.55 *Retention time and peak area are the average values derived from 8 individual runs of standard mixture of concentration - 10 mgL-1. **s is the standard deviation

Table 4: Repeatability of peak areas and retention times of a standard mixture of the dyes.