Parameter Method condition Rf % rsd of peak area DMP PCM TMD DMP PCM TMD Flow rate 0.9 ml/min 6.12 3.83 5.46 0.78 0.94 0.88 1.1 ml/min 4.18 2.96 3.68 0.45 0.79 0.68 Mobile phase ratio acetontrile/ Buffer 65 / 35 4.38 2.86 3.18 0.23 0.89 0.77 35 / 65 6.18 4.84 5.28 0.65 0.65 0.94

Table 4: Results from the robustness study of method.