| Validation Parameter |
Resolution between Impurity-A and Impurity-E |
| Flow rate (ml/min) |
| 1.4 |
2.61 |
| 1.5 |
2.52 |
| 1.6 |
2.39 |
| Column temperature (°C) |
| 25 |
2.54 |
| 30 |
2.65 |
Note: Changes by slight variation in flow rate and column temparature to ensure
whether resolution between impurity-A and Impurity-E remains unaffected. |
