| Method |
Pazufloxacin mesylate |
Cefoperazone sodium |
Sulbactam sodium |
| Concentration (µg/ml) |
SD (n=5) |
CV% |
%RE |
Concentration |
SD (n=5) |
CV% |
%RE |
Concentration |
SD (n=5) |
CV% |
%RE |
| RP-HPLC |
| Intra-day |
0.5 |
0.026 |
5.361 |
3.000 |
1 |
0.032 |
3.316 |
3.500 |
1 |
0.032 |
3.347 |
4.400 |
| |
1.5 |
0.050 |
3.358 |
0.333 |
3 |
0.037 |
1.222 |
0.433 |
3 |
0.040 |
1.342 |
0.133 |
| |
10 |
0.211 |
2.137 |
1.150 |
10 |
0.052 |
0.520 |
-0.250 |
10 |
0.064 |
0.643 |
0.350 |
| |
15 |
0.362 |
2.411 |
-0.100 |
25 |
0.621 |
2.572 |
3.424 |
20 |
0.302 |
1.509 |
-0.070 |
| Inter-day |
0.5 |
0.033 |
6.861 |
3.800 |
1 |
0.036 |
3.708 |
2.900 |
1 |
0.039 |
4.054 |
3.800 |
| |
1.5 |
0.050 |
3.333 |
1.00 |
3 |
0.084 |
2.801 |
-0.067 |
3 |
0.065 |
2.165 |
-0.700 |
| |
10 |
0.422 |
4.212 |
-0.220 |
10 |
0.062 |
0.625 |
0.460 |
10 |
0.075 |
0.743 |
-0.210 |
| |
15 |
0.382 |
2.568 |
0.827 |
25 |
0.702 |
2.806 |
-0.056 |
20 |
0.318 |
1.580 |
-0.605 |
| TLC |
|
|
|
|
|
|
|
|
|
|
|
|
| Intra-day |
1 |
0.054 |
6.034 |
10.500 |
6 |
0.306 |
5.442 |
6.283 |
4 |
0.265 |
6.650 |
0.375 |
| |
3 |
0.082 |
3.090 |
11.533 |
18 |
0.325 |
1.899 |
4.933 |
12 |
0.401 |
3.502 |
4.566 |
| |
12 |
0.526 |
4.627 |
5.267 |
20 |
0.411 |
2.103 |
2.300 |
16 |
0.326 |
2.036 |
-0.069 |
| |
18 |
0.713 |
4.203 |
5.750 |
23 |
0.631 |
2.853 |
3.826 |
19 |
1.020 |
5.403 |
0.632 |
| Inter-day |
1 |
0.052 |
5.781 |
9.700 |
6 |
0.312 |
5.835 |
10.883 |
4 |
0.302 |
7.900 |
4.425 |
| |
3 |
0.081 |
3.042 |
11.233 |
18 |
0.312 |
1.835 |
5.544 |
12 |
0.278 |
2.352 |
1.483 |
| |
12 |
0.152 |
1.346 |
5.875 |
20 |
0.562 |
2.964 |
5.210 |
16 |
0.468 |
2.938 |
0.438 |
| |
18 |
0.422 |
2.585 |
9.306 |
23 |
1.120 |
5.083 |
4.204 |
19 |
1.114 |
5.962 |
1.663 |
a-CV%: coefficient of variation
b-%RE: percent relative error |
