Pazufloxacin
Cefoperazone
Sulbactam sodium
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Freeze-thaw
0.5
0.489 ± 0.041
8.384
1
0.968 ± 0.0325
3.357
1
0.972 ± 0.036
3.704
1.5
1.498 ± 0.043
2.884
3
3.026 ± 0.0541
1.788
3
3.112 ± 0.0684
2.198
10
10.063 ± 0.302
3.001
10
9.962 ± 0.2011
2.019
10
9.985 ± 0.311
3.115
15
15.121 ± 0.355
2.348
25
30.062 ± 0.506
1.683
20
20.121 ± 0.663
3.295
Short term
0.5
0.482 ± 0.041
8.506
1
0.958 ± 0.041
4.279
1
0.976 ± 0.042
4.303
1.5
1.502 ± 0.046
3.036
3
2.984 ± 0.0622
2.084
3
3.022 ± 0.0552
1.827
10
9.996 ± 0.285
2.851
10
10.025 ± 0.2133
2.128
10
10.025 ± 0.338
3.372
15
14.933 ± 0.341
2.284
25
30.018 ± 0.522
1.739
20
20.021 ± 0.625
3.122
Long term
0.5
0.486 ± 0.038
7.819
1
0.978 ± 0.036
3.681
1
0.952 ± 0.053
5.567
1.5
1.493 ± 0.043
2.908
3
3.112 ± 0.033
1.067
3
2.966 ± 0.0632
2.131
10
9.889 ± 0.233
2.356
10
9.912 ± 0.196
1.982
10
9.954 ± 0.328
3.295
15
15.021 ± 0.411
2.736
25
29.932 ± 0.814
2.719
20
19.912 ± .744
3.736
Post preparative
0.5
0.481 ± 0.036
7.484
1
0.953 ± 0.045
4.722
1
0.974 ± 0.038
3.901
1.5
1.500 ± 0.051
3.406
3
2.995 ± 0.0415
1.386
3
2.984 ± 0.0521
1.746
10
9.984 ± 0.195
1.953
10
10.062 ± 0.1652
1.642
10
10.163 ± 0.203
1.997
15
14.965 ± 0.406
2.713
25
30.052± 0.425
1.414
25
20.012 ± 0.741
3.703
a-* Mean of five determinations
Table 3
: Stability study of Pazufloxacin, Cefoperazone and Sulbactam sodium in human plasma by the proposed HPLC method.
