Pazufloxacin
Cefoperazone
Sulbactam sodium
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Plasma concentration (µg/ml)
Found* (Mean ± SD)
CV%
Freeze-thaw
1
0.932 ± 0.043
4.614
6
5.448 ± 0.365
6.700
4
3.741 ± 0.204
5.453
3
2.847 ± 0.068
2.388
18
17.996 ± 0.503
2.795
12
11.484 ± 0.336
2.926
12
11.896 ± 0.332
2.791
20
19.598 ± 0.632
3.225
16
15.658 ± 0.516
3.295
18
16.984 ± 0.685
4.033
23
21.814 ± 0.762
3.507
19
18.365 ± 0.388
2.113
Short term
1
0.941 ± 0.038
4.038
6
5.865 ± 0.385
6.564
4
3.899 ± 0.196
5.027
3
2.871 ± 0.071
2.473
18
17.652 ± 0.416
2.357
12
12.006 ± 0.425
3.540
12
11.445 ± 0.205
1.791
20
19.852 ± 0.811
4.085
16
15.741 ± 0.602
3.824
18
17.062 ± 0.625
3.663
23
21.745 ± 0.698
3.210
19
19.012 ± 0.374
1.967
Long term
1
0.925 ± 0.051
5.514
6
5.462 ± 0.395
7.232
4
3.602 ± 0.216
5.997
3
2.762 ± 0.074
2.679
18
17.062 ± 0.541
3.171
12
11.836 ± 0.325
2.746
12
11.303 ± 0.217
1.920
20
18.326 ± 0.506
2.761
16
15.322 ± 0.425
2.774
18
17.622 ± 0.387
2.196
23
21.881 ± 0.852
3.894
19
18.385 ± 0.811
4.411
Post preparative
1
0.957 ± 0.045
4.702
6
5.841 ± 0.398
6.814
4
3.902 ± 0.215
5.510
3
2.911 ± 0.065
2.233
18
17.124 ± 0.526
3.072
12
12.001 ± 0.347
2.891
12
11.395 ± 0.122
1.071
20
19.629 ± 0.805
4.101
16
15.877 ± 0.285
1.795
18
17.818 ± 0.528
2.963
23
22.001 ± 1.022
4.645
19
18.625 ± 0.502
2.695
a- * Mean of five determinations
Table 4
: Stability study of Pazufloxacin, Cefoperazone and Sulbactam sodium in human plasma by the proposed TLC method.
