Parameter
BF
HCTZ
Linearity range (ng/ spot)
200–1200
100–800
Correlation coefficient
0.9991
0.9989
Limit of detection (ng/spot)
34.59
16.2530.26
Limit of quantitation (ng/spot)
104.54326.62
50.0991.71
% Recovery (n=9)
99.71
100.08
Ruggedness (% R.S.D.)
Analyst I ( n=3)
1.19
0.95
Analyst II (n=3)
0.71
Precision (%R.S.D.)
Repeatability of application (n=6)
1.63
0.910.84
Intra-day (n=3)
0.28–0.94
0.46–0.61
Inter-day (n=3)
0.45–1.19
1.19–0.83
Robustness
Robust
Specificity
Specific
