pH Dm Rr R T
3 0.35 1.35 5.69 1.17
4 0.28 1.28 4.62 1.36
5 0.46 1.46 6.71 1.07
6 0.51 1.51 7.07 1.27
Dm is the mass distribution ratio [also known as the capacity factor (K`) or the retention factor (K)].
Rr is the relative retention times [also known as the unadjusted relative retention (rG)].
R is the resolution.
T is the tailing factor [also known as the symmetry factor (As)].
Table 2: Effect of pH of phosphate buffer on the chromatographic performance of the HPLC method.