Buffer concentration Dm Rr R T 0.005 M 0.56 1.68 7.52 1.32 0.01 M 0.44 1.47 6.60 1.33 0.02 M 0.20 1.03 3.64 1.11 0.03 M 0.18 1.01 3.18 1.18 0.04 M 0.14 0.94 2.54 1.15 0.05 M 0.14 0.93 2.72 1.19 0.06 M 0.13 0.91 2.54 1.19 Dm is the mass distribution ratio [also known as the capacity factor (K`) or the retention factor (K)]. Rr is the relative retention times [also known as the unadjusted relative retention (rG)]. R is the resolution. T is the tailing factor [also known as the symmetry factor (As)].

Table 3: Effect of concentration of phosphate buffer on the chromatographic performance of the HPLC method.