Flow rate (mL/min) Dm Rr R T
0.5 0.20 1.03 3.41 1.18
0.8 0.21 1.03 3.81 1.15
1.0 0.21 1.03 3.65 1.11
1.2 0.20 1.02 3.27 1.09
1.5 0.19 0.97 2.79 1.05
Dm is the mass distribution ratio [also known as the capacity factor (K`) or the retention factor (K)].
Rr is the relative retention times [also known as the unadjusted relative retention (rG)].
R is the resolution.
T is the tailing factor [also known as the symmetry factor (As)].
Table 4: Effect of flow rate on the chromatographic performance of the HPLC method.