Table 4

Flow rate (mL/min)	*D_m*	*R_r*	R	T
0.5	0.20	1.03	3.41	1.18
0.8	0.21	1.03	3.81	1.15
1.0	0.21	1.03	3.65	1.11
1.2	0.20	1.02	3.27	1.09
1.5	0.19	0.97	2.79	1.05

D_m is the mass distribution ratio [also known as the capacity factor (K`) or the retention factor (K)].
R_r is the relative retention times [also known as the unadjusted relative retention (r_G)].
R is the resolution.
T is the tailing factor [also known as the symmetry factor (A_s)].

Table 4: Effect of flow rate on the chromatographic performance of the HPLC method.