| Column |
Stainless Steel Merck 250
C8 Column, 5 µm
(250 mm×4.6 mm i.d.) |
| Mobile phase |
Acetonitrile:Methanol:0.02M Phosphate buffer (pH 5)
[20:20:60] |
| Flow
rate |
1 mL/min. |
| Detector |
UV-detection at 226nm |
| Injection
volume |
20 µL |
| Temp. |
Ambient |
| Internal
Standard |
Pindolol |
|
