Column Stainless Steel Merck 250 C8 Column, 5 µm (250 mm×4.6 mm i.d.)
Mobile phase Acetonitrile:Methanol:0.02M Phosphate buffer (pH 5) [20:20:60]
Flow rate 1 mL/min.
Detector UV-detection at 226nm
Injection volume 20 µL
Temp. Ambient
Internal Standard Pindolol
Table 5: Typical chromatographic conditions for the HPLC determination of atenolol.