Parameters MET REPA
Range (ng/band) 500-2500 100-500
Tailing factor 1.46 1.31
Detection limit (ng/band) 98 17
Quantitation limit (ng/band) 296 51
Accuracy (%) 98.35-101.76 % 97.85-100.78%
  Precision (%RSD)
Intra-day (n=3) 0.16-0.33 0.89-1.96
Inter-day (n=3) 0.14 -0.50 0.63-1.76
Instrument precision  (%RSD) 0.16 0.89
Specificity Specific Specific
Table 3: Summary of validation parameters of developed HPTLC method.